Application
E2F7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the E2F7 antibody detected the E2F7 protein (arrow).| Product Name | E2F7 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F7; Transcription factor E2F7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | E2F7 Transcription factor E2F7 |
|---|---|
| Molecular Weight(MW) | 99888 Da |
| Function | Atypical E2F transcription factor that participates to various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'- TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1. Acts as a regulator of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-3' and mediating repression of G1/S-regulated genes. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Also involved in DNA damage response: up-regulated by p53/TP53 following genotoxic stress and acts as a downstream effector of p53/TP53-dependent repression by mediating repression of indirect p53/TP53 target genes involved in DNA replication. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. Acts as a negative regulator of keratinocyte differentiation |
| Tissue Specificity | This E2F7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-619 amino acids from the Central region of human E2F7. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 144455 |
|---|
Application
E2F7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the E2F7 antibody detected the E2F7 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
