Application
Western blot analysis of EBI3 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. EBI3 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).| Product Name | EBI3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EBI3; IL27B; Interleukin-27 subunit beta; Epstein-Barr virus-induced gene 3 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL27B |
|---|---|
| Alternative Names | EBI3 IL27B Interleukin-27 subunit beta Epstein-Barr virus-induced gene 3 protein |
| Molecular Weight(MW) | 25396 Da |
| Function | Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines |
| Tissue Specificity | This EBI3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-181 amino acids from the C-terminal region of human EBI3. |
| Cellular Localization | Secreted. |
| Entrez Gene | 10148 |
|---|---|
| Nucleotide Accession | NP_005746.2 |
Application
Western blot analysis of EBI3 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. EBI3 Antibody (C-term) (arrow) was detected using the purified Pab (1:60/250 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
