Western blot analysis of ECE-1 Antibody (Center) in Y79(lane 1), T47D(lane 2) cell line and mouse lung(lane 3), liver(lane 4) tissue lysates (35ug/lane). ECE-1 (arrow) was detected using the purified Pab.
All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney
Cellular Localization
Cell membrane; Single-pass type II membrane protein
Western blot analysis of ECE-1 Antibody (Center) in Y79(lane 1), T47D(lane 2) cell line and mouse lung(lane 3), liver(lane 4) tissue lysates (35ug/lane). ECE-1 (arrow) was detected using the purified Pab.
Application Notes
WB:1:100~500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.