Application
EDN1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EDN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EDN1 Antibody (C-term) western blot analysis in ZR-75-1,U251,MDA-MB453,A549 cell line lysates (35ug/lane).This demonstrates the EDN1 antibody detected the EDN1 protein (arrow).Application
Confocal immunofluorescent analysis of EDN1 Antibody (C-term)(Cat#AP11973b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | EDN1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EDN1; Endothelin-1; Preproendothelin-1; Big endothelin-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EDN1 Endothelin-1 Preproendothelin-1 Big endothelin-1 |
|---|---|
| Molecular Weight(MW) | 24425 Da |
| Function | Endothelins are endothelium-derived vasoconstrictor peptides |
| Tissue Specificity | Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells |
| Cellular Localization | Secreted. |
Application
EDN1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EDN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EDN1 Antibody (C-term) western blot analysis in ZR-75-1,U251,MDA-MB453,A549 cell line lysates (35ug/lane).This demonstrates the EDN1 antibody detected the EDN1 protein (arrow).
Application
Confocal immunofluorescent analysis of EDN1 Antibody (C-term)(Cat#AP11973b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
