Application
A. Western blot analysis on Hek293 cell lysate of using Anti-eEF2k (C-term) Rabbit Monoclonal (Cat. #AJ1251b), dilution 1:1000.| Product Name | eEF-2K Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP881Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EEF2K Elongation factor 2 kinase Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
|---|---|
| Molecular Weight(MW) | 82144 Da |
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced |
| Tissue Specificity | A synthetic peptide corresponding to residues in the C-terminal of human eEF2 was used as an immunogen. |
| Entrez Gene | 29904 |
|---|
Application
A. Western blot analysis on Hek293 cell lysate of using Anti-eEF2k (C-term) Rabbit Monoclonal (Cat. #AJ1251b), dilution 1:1000.| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
