Application
Western blot analysis of anti-EEF2K in HepG2 cell line lysate (35ug/lane).EEF2K(arrow) was detected using the purified Pab (1:60 dilution).| Product Name | EEF2K Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EEF2K Eukaryotic elongation factor 2 kinase Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
|---|---|
| Molecular Weight(MW) | 82144 Da |
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced |
| Tissue Specificity | This EEF2K antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~65 amino acids from the N-term of human elongation factor 2 kinase. |
Application
Western blot analysis of anti-EEF2K in HepG2 cell line lysate (35ug/lane).EEF2K(arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
