Application
Western blot analysis of EEFSEC Antibody (C-term) in Hela cell line lysates (35ug/lane). EEFSEC (arrow) was detected using the purified Pab.Application
EEFSEC Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EEFSEC Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EEFSEC Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EEFSEC Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SELB |
|---|---|
| Alternative Names | EEFSEC SELB Selenocysteine-specific elongation factor Elongation factor sec Eukaryotic elongation factor selenocysteine-tRNA-specific |
| Molecular Weight(MW) | 65305 Da |
| Function | Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP |
| Tissue Specificity | This EEFSEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 553~582 amino acids from the C-terminal region of human EEFSEC. |
| Cellular Localization | Cytoplasm (Potential). Nucleus (Potential). |
| Entrez Gene | 60678 |
|---|
Application
Western blot analysis of EEFSEC Antibody (C-term) in Hela cell line lysates (35ug/lane). EEFSEC (arrow) was detected using the purified Pab.
Application
EEFSEC Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EEFSEC Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EEFSEC Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
