Application
EFNB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EFNB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EFNB2 Antibody (Center) western blot analysis in Hela,293 cell line lysates (35ug/lane).This demonstrates the EFNB2 antibody detected the EFNB2 protein (arrow).Application
Confocal immunofluorescent analysis of EFNB2 Antibody (Center)(Cat#AP11652c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | EFNB2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EPLG5, HTKL, LERK5 |
|---|---|
| Alternative Names | EFNB2 EPLG5 HTKL LERK5 Ephrin-B2 EPH-related receptor tyrosine kinase ligand 5 HTK ligand |
| Molecular Weight(MW) | 36923 Da |
| Function | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons |
| Tissue Specificity | Lung and kidney. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 1948 |
|---|---|
| Nucleotide Accession | NP_004084.1 |
Application
EFNB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EFNB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EFNB2 Antibody (Center) western blot analysis in Hela,293 cell line lysates (35ug/lane).This demonstrates the EFNB2 antibody detected the EFNB2 protein (arrow).
Application
Confocal immunofluorescent analysis of EFNB2 Antibody (Center)(Cat#AP11652c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
