Application
EGFR Antibody (C-term)(Ascites)(Cat. #AM2179a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EGFR antibody detected the EGFR protein (arrow).| Product Name | EGFR Antibody (C-term) (Ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 688CT33.1.3 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100~200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERBB, ERBB1, HER1 |
|---|---|
| Alternative Names | EGFR ERBB ERBB1 HER1 Epidermal growth factor receptor Proto-oncogene c-ErbB-1 Receptor tyrosine-protein kinase erbB-1 |
| Molecular Weight(MW) | 134277 Da |
| Function | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin |
| Tissue Specificity | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand |
| Entrez Gene | 1956 |
|---|---|
| Nucleotide Accession | NP_005219.2 |
Application
EGFR Antibody (C-term)(Ascites)(Cat. #AM2179a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EGFR antibody detected the EGFR protein (arrow).| Application Notes | WB:1:100~200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
