Application
Western blot analysis of EHD3 Antibody (Center) in CEM cell line lysates (35ug/lane). EHD3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of 293 cells using EHD3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EHD3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EHD2, PAST3 |
|---|---|
| Alternative Names | EHD3 EHD2 PAST3 EH domain-containing protein 3 PAST homolog 3 |
| Molecular Weight(MW) | 60887 Da |
| Function | Plays a role in endocytic transport. |
| Tissue Specificity | Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein Recycling endosome membrane; Peripheral membrane protein Cytoplasm (By similarity) |
| Entrez Gene | 30845 |
|---|
Application
Western blot analysis of EHD3 Antibody (Center) in CEM cell line lysates (35ug/lane). EHD3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of 293 cells using EHD3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
