Application
A. Western blot analysis on 293T cell lysates using anti-Phospho-eIF4B (pS504) RabMAb (Cat. #AJ1254b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with AP.| Product Name | eIF-4B Antibody Phospho (pS504) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S504 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2261Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4B Eukaryotic translation initiation factor 4B |
|---|---|
| Molecular Weight(MW) | 69151 Da |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B was used as an immunogen. This antibody detects eIF4B phosphorylated at serine 504. |
| Entrez Gene | 1975 |
|---|
Application
A. Western blot analysis on 293T cell lysates using anti-Phospho-eIF4B (pS504) RabMAb (Cat. #AJ1254b), 1:50,000 dilution. Cells were either (A) untreated (B) treated with AP.| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
