Application
A. Western blot analysis on Jurkat cell lysates using anti-eIF-5A RabMAb (Cat. #AJ1256b), 1:1,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti-eIF-5alpha RabMAb (Cat. #AJ1256b).| Product Name | eIF-5A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP527Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF5A Eukaryotic translation initiation factor 5A-1 eIF-4D Rev-binding factor |
|---|---|
| Molecular Weight(MW) | 16832 Da |
| Function | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts |
| Tissue Specificity | Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level) |
| Cellular Localization | Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions |
| Entrez Gene | 1984 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-eIF-5A RabMAb (Cat. #AJ1256b), 1:1,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti-eIF-5alpha RabMAb (Cat. #AJ1256b).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
