Application
EIF2C2 Antibody (N-term Q41) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2C2 antibody detected the EIF2C2 protein (arrow).Application
EIF2C2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2C2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EIF2C2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EIF2C2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Ago2, Kiaa4215 |
|---|---|
| Alternative Names | Eif2c2 Ago2 Kiaa4215 Protein argonaute-2 Eukaryotic translation initiation factor 2C 2 Piwi/argonaute family protein meIF2C2 Protein slicer |
| Molecular Weight(MW) | 97304 Da |
| Function | Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC- mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7- methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). May also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. May also be required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. Regulates lymphoid and erythroid development and function, and this is independent of endonuclease activity |
| Tissue Specificity | Ubiquitous expression in 9.5 day embryos with highest levels in forebrain, heart, limb buds, and branchial arches |
| Cellular Localization | Cytoplasm, P-body (By similarity). Nucleus (By similarity). Note=Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8 (By similarity) |
| Entrez Gene | 239528 |
|---|
Application
EIF2C2 Antibody (N-term Q41) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF2C2 antibody detected the EIF2C2 protein (arrow).
Application
EIF2C2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2C2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EIF2C2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.10% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
