Application
EIF3A Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the EIF3A antibody detected the EIF3A protein (arrow).Application
EIF3A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | EIF3A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF3S10, KIAA0139 |
|---|---|
| Alternative Names | EIF3A EIF3S10 KIAA0139 Eukaryotic translation initiation factor 3 subunit A Eukaryotic translation initiation factor 3 subunit 10 eIF-3-theta eIF3 p167 eIF3 p180 eIF3 p185 |
| Molecular Weight(MW) | 166569 Da |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation |
| Tissue Specificity | This EIF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1218-1247 amino acids from the C-terminal region of human EIF3A. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 8661 |
|---|---|
| Nucleotide Accession | NP_003741.1 |
Application
EIF3A Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the EIF3A antibody detected the EIF3A protein (arrow).
Application
EIF3A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
