Application
EIF4B Antibody (pS498) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).Application
Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (pS498) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4B gene.Application
EIF4B Antibody (Center S498) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4B Antibody (Center S498) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EIF4B Antibody (Center S498) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EIF4B Antibody (Center S498) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4B Eukaryotic translation initiation factor 4B |
|---|---|
| Molecular Weight(MW) | 69151 Da |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F |
| Tissue Specificity | This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Central region of human EIF4B. |
| Entrez Gene | 1975 |
|---|---|
| Nucleotide Accession | NP_001408.2 |
Application
EIF4B Antibody (pS498) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).
Application
Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (pS498) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4B gene.
Application
EIF4B Antibody (Center S498) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4B Antibody (Center S498) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EIF4B Antibody (Center S498) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
