Application
Western blot analysis of anti-EIF4B Antibody (Center) Pab in HepG2 cell line lysates (35ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of anti-EIF4B Antibody (Center) Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EIF4B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4B Eukaryotic translation initiation factor 4B |
|---|---|
| Molecular Weight(MW) | 69151 Da |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F |
| Tissue Specificity | This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360~390 amino acids from the center region of human EIF4B Antibody. |
Application
Western blot analysis of anti-EIF4B Antibody (Center) Pab in HepG2 cell line lysates (35ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of anti-EIF4B Antibody (Center) Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane). EIF4B(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
