Application
Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (S422) (RB13724). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B-S422, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EIF4B Antibody (S422) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4B Eukaryotic translation initiation factor 4B |
|---|---|
| Molecular Weight(MW) | 69151 Da |
| Function | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F |
| Tissue Specificity | This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S422 of human EIF4B. |
Application
Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (S422) (RB13724). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B-S422, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
