Application
Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4EBP1 gene.Application
EIF4EBP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EIF4EBP1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EIF4EBP1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4EBP1 Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
|---|---|
| Molecular Weight(MW) | 12580 Da |
| Function | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways |
| Tissue Specificity | This EIF4EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-69 amino acids from the Central region of human EIF4EBP1. |
| Entrez Gene | 1978 |
|---|---|
| Nucleotide Accession | NP_004086.1 |
Application
Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4EBP1 gene.
Application
EIF4EBP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EIF4EBP1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
