Application
Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (S100) (RB11410). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4EBP1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with EIF4EBP1 Antibody (S100) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EIF4EBP1 Antibody (S100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF4EBP1 Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
|---|---|
| Molecular Weight(MW) | 12580 Da |
| Function | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways |
| Tissue Specificity | This EIF4EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S100 of human EIF4EBP1. |
Application
Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (S100) (RB11410). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4EBP1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with EIF4EBP1 Antibody (S100) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
