Application
Western blot analysis of EIF5A Antibody (Center) in MDA-MB231 and MCF-7 cell line lysates (35ug/lane). EIF5A (arrow) was detected using the purified Pab.Application
Western blot analysis of EIF5A (arrow) using rabbit polyclonal EIF5A Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF5A gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with EIF5A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EIF5A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF5A; Eukaryotic translation initiation factor 5A-1; Eukaryotic initiation factor 5A isoform 1; Rev-binding factor; eIF-4D |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF5A Eukaryotic translation initiation factor 5A-1 Eukaryotic initiation factor 5A isoform 1 Rev-binding factor eIF-4D |
|---|---|
| Molecular Weight(MW) | 16832 Da |
| Function | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts |
| Tissue Specificity | Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level) |
| Cellular Localization | Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions |
| Entrez Gene | 1984 |
|---|
Application
Western blot analysis of EIF5A Antibody (Center) in MDA-MB231 and MCF-7 cell line lysates (35ug/lane). EIF5A (arrow) was detected using the purified Pab.
Application
Western blot analysis of EIF5A (arrow) using rabbit polyclonal EIF5A Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF5A gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with EIF5A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
