Application
EIF5AL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF5AL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EIF5AL1 Antibody (C-term) western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).This demonstrates the EIF5AL1 antibody detected the EIF5AL1 protein (arrow).Application
Confocal immunofluorescent analysis of EIF5AL1 Antibody (C-term)(Cat#AP11986b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | EIF5AL1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF5AL1; Eukaryotic translation initiation factor 5A-1-like; Eukaryotic initiation factor 5A isoform 1-like |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EIF5AL1 Eukaryotic translation initiation factor 5A-1-like Eukaryotic initiation factor 5A isoform 1-like |
|---|---|
| Molecular Weight(MW) | 16773 Da |
| Function | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity) |
| Tissue Specificity | This EIF5AL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-130 amino acids from the C-terminal region of human EIF5AL1. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Nucleus, nuclear pore complex (By similarity). Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (By similarity) |
| Entrez Gene | 143244 |
|---|---|
| Nucleotide Accession | NP_001093162.1 |
Application
EIF5AL1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF5AL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EIF5AL1 Antibody (C-term) western blot analysis in Ramos,A2058 cell line lysates (35ug/lane).This demonstrates the EIF5AL1 antibody detected the EIF5AL1 protein (arrow).
Application
Confocal immunofluorescent analysis of EIF5AL1 Antibody (C-term)(Cat#AP11986b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
