Application
ELAVL1/MB10217 antibody (Cat. #AM1899b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ELAVL1/MB10217 antibody detected the ELAVL1/MB10217 protein (arrow).Application
Confocal immunofluorescent analysis of ELAVL1 Antibody (Cat#AM1899b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ELAVL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 224CT6.5.3 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~1000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HUR |
|---|---|
| Alternative Names | ELAVL1 HUR ELAV-like protein 1 Hu-antigen R |
| Molecular Weight(MW) | 36092 Da |
| Function | Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2 |
| Entrez Gene | 1994 |
|---|---|
| Nucleotide Accession | NP_001410.2 |
Application
ELAVL1/MB10217 antibody (Cat. #AM1899b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ELAVL1/MB10217 antibody detected the ELAVL1/MB10217 protein (arrow).
Application
Confocal immunofluorescent analysis of ELAVL1 Antibody (Cat#AM1899b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~1000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
