Application
Western blot analysis of anti-ELF4 Antibody (Center) in 293 cell line lysates (35ug/lane). ELF4 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ELF4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ELF4; ELFR; MEF; ETS-related transcription factor Elf-4; E74-like factor 4; Myeloid Elf-1-like factor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ELFR MEF |
|---|---|
| Alternative Names | ELF4 ELFR MEF ETS-related transcription factor Elf-4 E74-like factor 4 Myeloid Elf-1-like factor |
| Molecular Weight(MW) | 70730 Da |
| Function | Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner Can also promote cellular transformation through inhibition of the p16 pathway |
| Tissue Specificity | Abundantly expressed in the placenta and in a variety of myeloid leukemia cell lines. Moderate levels of expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary and colon. Lower levels of expression in Jurkat T-cells and other T-cell lines and no expression in brain |
| Cellular Localization | Nucleus, PML body. Note=Accumulation into PML nuclear bodies is mediated by PML |
| Entrez Gene | 2000 |
|---|
Application
Western blot analysis of anti-ELF4 Antibody (Center) in 293 cell line lysates (35ug/lane). ELF4 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
