Application
ELL3 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ELL3 antibody detected the ELL3 protein (arrow).| Product Name | ELL3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ELL3; RNA polymerase II elongation factor ELL3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ELL3 RNA polymerase II elongation factor ELL3 |
|---|---|
| Molecular Weight(MW) | 45361 Da |
| Function | Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P-TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT) (By similarity). Elongation factors can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA |
| Tissue Specificity | Testis specific. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 80237 |
|---|---|
| Nucleotide Accession | NP_079441.1 |
Application
ELL3 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ELL3 antibody detected the ELL3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
