Application
Fluorescent confocal image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2884b ELP2 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).Application
Western blot analysis of ELP2 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). ELP2 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ELP2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ELP2; STATIP1; Elongator complex protein 2; SHINC-2; STAT3-interacting protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBFC |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | STATIP1 |
|---|---|
| Alternative Names | ELP2 STATIP1 Elongator complex protein 2 SHINC-2 STAT3-interacting protein 1 |
| Molecular Weight(MW) | 92500 Da |
| Function | Regulates the ligand-dependent activation of STAT3 (By similarity) |
| Tissue Specificity | This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744~773 amino acids from the C-terminal region of human ELP2. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 55250 |
|---|
Application
Fluorescent confocal image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP2884b ELP2 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).
Application
Western blot analysis of ELP2 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). ELP2 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
