Application
"Western blot analysis of EME1 (arrow) using rabbit polyclonal EME1 Antibody(Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EME1 gene (Lane 2) (Origene Technologies)."| Product Name | EME1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EME1; MMS4; Crossover junction endonuclease EME1; MMS4 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MMS4 |
|---|---|
| Alternative Names | EME1 MMS4 Crossover junction endonuclease EME1 MMS4 homolog |
| Molecular Weight(MW) | 63252 Da |
| Function | Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions May be required in mitosis for the processing of stalled or collapsed replication forks |
| Tissue Specificity | This EME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288~318 amino acids from the Center region of human EME1. |
| Cellular Localization | Nucleus, nucleolus. Note=Recruited to regions of DNA damage in S-phase cells |
| Entrez Gene | 146956 |
|---|
Application
"Western blot analysis of EME1 (arrow) using rabbit polyclonal EME1 Antibody(Center) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EME1 gene (Lane 2) (Origene Technologies)."| Application Notes | WB:1:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
