Application
EMG1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EMG1 antibody detected the EMG1 protein (arrow).Application
EMG1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EMG1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EMG1; C2F; Ribosomal RNA small subunit methyltransferase NEP1; 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; 18S rRNA Psi1248 methyltransferase; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C2F |
|---|---|
| Alternative Names | EMG1 C2F Ribosomal RNA small subunit methyltransferase NEP1 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1 18S rRNA Psi1248 methyltransferase Nucleolar protein EMG1 homolog Protein C2f Ribosome biogenesis protein NEP1 |
| Molecular Weight(MW) | 26720 Da |
| Function | Involved in 40S ribosomal subunit biogenesis and 18S rRNA processing. Specifically catalyzes the N1-methylation of pseudouridine at position 1248 (Psi1248) in 18S rRNA. Thus, appears to be the methyltransferase involved in the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) in position 1248 in 18S rRNA. Is not able to methylate uridine at this position |
| Tissue Specificity | This EMG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the Central region of human EMG1. |
| Cellular Localization | Nucleus, nucleolus. |
| Entrez Gene | 10436 |
|---|---|
| Nucleotide Accession | NP_006322.4 |
Application
EMG1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EMG1 antibody detected the EMG1 protein (arrow).
Application
EMG1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
