Application
Western blot analysis of EN2 antibody (C-term) (Cat.# AP7457b) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.Application
Fluorescent confocal image of Hela cell stained with EN2 Antibody (C-term)(Cat#AP7457b). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.| Product Name | EN2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EN2; Homeobox protein engrailed-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EN2 Homeobox protein engrailed-2 |
|---|---|
| Molecular Weight(MW) | 34211 Da |
| Tissue Specificity | This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250~279 amino acids from the C-terminal region of human EN2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2020 |
|---|
Application
Western blot analysis of EN2 antibody (C-term) (Cat.# AP7457b) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.
Application
Fluorescent confocal image of Hela cell stained with EN2 Antibody (C-term)(Cat#AP7457b). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
