Application
Western blot analysis of EN2 antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human brain reacted with EN2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using EN2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EN2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EN2; Homeobox protein engrailed-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EN2 Homeobox protein engrailed-2 |
|---|---|
| Molecular Weight(MW) | 34211 Da |
| Tissue Specificity | This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64~93 amino acids from the N-terminal region of human EN2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2020 |
|---|
Application
Western blot analysis of EN2 antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human brain reacted with EN2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using EN2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
