Application
ENASE Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ENASE antibody detected the ENASE protein (arrow).Application
ENASE Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ENASE Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ENASE Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENGASE; Cytosolic endo-beta-N-acetylglucosaminidase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ENGASE Cytosolic endo-beta-N-acetylglucosaminidase |
|---|---|
| Molecular Weight(MW) | 83987 Da |
| Function | Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol |
| Tissue Specificity | Widely expressed. Expressed at higher level in thymus and spleen |
| Cellular Localization | Cytoplasm, cytosol (By similarity). |
| Entrez Gene | 64772 |
|---|---|
| Nucleotide Accession | NP_001036038.1 |
Application
ENASE Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ENASE antibody detected the ENASE protein (arrow).
Application
ENASE Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ENASE Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
