Application
Western blot analysis of ENDOG Antibody (C-term) (Cat. 3AP6571b) in 293,K562,Jurkat,NCI-H460 cell line lysates and mouse kidney tissues lysates(35ug/lane). ENDOG (arrow) was detected using the purified Pab.Application
ENDOG Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ENDOG Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENDOG; Endonuclease G, mitochondrial |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ENDOG Endonuclease G mitochondrial |
|---|---|
| Molecular Weight(MW) | 32620 Da |
| Function | Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity) |
| Tissue Specificity | This ENDOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195~225 amino acids from the C-terminal region of human ENDOG. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 2021 |
|---|
Application
Western blot analysis of ENDOG Antibody (C-term) (Cat. 3AP6571b) in 293,K562,Jurkat,NCI-H460 cell line lysates and mouse kidney tissues lysates(35ug/lane). ENDOG (arrow) was detected using the purified Pab.
Application
ENDOG Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
