Application
A. Western blot analysis on (A) fetal brain, (B) SH-SY-5Y, (C) HeLa and (D) Y79 cell lysates using anti-Enolase 2 gamma (NSE) RabMAb (Cat. #AJ1261a), dilution: 1:20,000.Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Enolase 2 gamme (NSE) RabMAb (Cat. #AJ1261a).| Product Name | Enolase-2 Antibody gamma (NSE) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENO2, Gamma-enolase, Enolase 2 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3377 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ENO2 Gamma-enolase Enolase 2 |
|---|---|
| Molecular Weight(MW) | 47269 Da |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity) |
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity) |
| Entrez Gene | 2026 |
|---|
Application
A. Western blot analysis on (A) fetal brain, (B) SH-SY-5Y, (C) HeLa and (D) Y79 cell lysates using anti-Enolase 2 gamma (NSE) RabMAb (Cat. #AJ1261a), dilution: 1:20,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Enolase 2 gamme (NSE) RabMAb (Cat. #AJ1261a).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
