Application
Western blot analysis of ENPP6 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane).ENPP6 (arrow) was detected using the purified Pab.Application
ENPP6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENPP6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ENPP6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ENPP6; Ectonucleotide pyrophosphatase/phosphodiesterase family member 6; Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 soluble form |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ENPP6 Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 soluble form |
|---|---|
| Molecular Weight(MW) | 50241 Da |
| Function | Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin (By similarity). Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'- monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid |
| Tissue Specificity | Predominantly expressed in kidney and brain. In the kidney, expressed specifically in the proximal tubules and thin descending limbs of Henle (at protein level) |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Note=A small amount of the protein may be found in the extracellular milieu |
| Entrez Gene | 133121 |
|---|
Application
Western blot analysis of ENPP6 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane).ENPP6 (arrow) was detected using the purified Pab.
Application
ENPP6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENPP6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
