Application
Western blot analysis of ENTH Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENTH (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ENTH Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ENTH, EPN4, EPNR, KIAA0171 |
|---|---|
| Alternative Names | CLINT1 ENTH EPN4 EPNR KIAA0171 Clathrin interactor 1 Clathrin-interacting protein localized in the trans-Golgi region Enthoprotin Epsin-4 Epsin-related protein |
| Molecular Weight(MW) | 68259 Da |
| Function | Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly |
| Tissue Specificity | Ubiquitously expressed at low to intermediate levels |
| Cellular Localization | Cytoplasm. Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network |
| Entrez Gene | 9685 |
|---|
Application
Western blot analysis of ENTH Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENTH (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human brain with ENTH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ENTH Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
