Application
Western blot analysis of ENPP2 (arrow) using rabbit polyclonal ENPP2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ENPP2 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of ENPP2 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). ENPP2 (arrow) was detected using the purified Pab.Application
ENPP2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ENPP2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATX, PDNP2 |
|---|---|
| Alternative Names | ENPP2 ATX PDNP2 Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 Autotaxin Extracellular lysophospholipase D |
| Molecular Weight(MW) | 98994 Da |
| Function | Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor |
| Tissue Specificity | Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues |
| Cellular Localization | Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells |
| Entrez Gene | 5168 |
|---|
Application
Western blot analysis of ENPP2 (arrow) using rabbit polyclonal ENPP2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ENPP2 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of ENPP2 Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). ENPP2 (arrow) was detected using the purified Pab.
Application
ENPP2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
