Application
Figure 1: Western blot analysis using anti-EP300 monoclonal antibody against truncated EP300-His recombinant protein(1).| Product Name | EP300 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p300; KAT3B |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7D8A6 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P300 |
|---|---|
| Alternative Names | p300 KAT3B |
| Molecular Weight(MW) | 264161 Da |
| Function | Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity |
| Tissue Specificity | Purified recombinant fragment of EP300 expressed in E. Coli. |
| Cellular Localization | Cytoplasm. Nucleus. Note=In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Co-localizes with ROCK2 in the nucleus |
| Entrez Gene | 2033 |
|---|---|
| Nucleotide Accession | NP_001420.2 |
Application
Figure 1: Western blot analysis using anti-EP300 monoclonal antibody against truncated EP300-His recombinant protein(1).| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of EP300 expressed in E. Coli. |
