Application
A. Western blot analysis on A431 cell lysate using anti-Epithelial Antigen (Ep-CAM) (C-term) RabMAb (Cat. #AJ1263a); dilution 1:2,500.Application
B. Immunohistochemical analysis of paraffin-embedded stomach carcinoma using anti-Epithelial Antigen (Ep-CAM) (C-term) RabMAb (Cat. #AJ1263a).| Product Name | Epcam Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E144 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GA733-2, M1S2, M4S1, MIC18, TACSTD1, TRO |
|---|---|
| Alternative Names | EPCAM GA733-2 M1S2 M4S1 MIC18 TACSTD1 TROP1 Epithelial cell adhesion molecule Adenocarcinoma-associated antigen KSA KS 1/4 antigen Cell surface glycoprotein Trop-1 |
| Molecular Weight(MW) | 34932 Da |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E |
| Tissue Specificity | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma |
| Cellular Localization | Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Co-localizes with CLDN7 at the lateral cell membrane and tight junction |
| Entrez Gene | 4072 |
|---|
Application
A. Western blot analysis on A431 cell lysate using anti-Epithelial Antigen (Ep-CAM) (C-term) RabMAb (Cat. #AJ1263a); dilution 1:2,500.
Application
B. Immunohistochemical analysis of paraffin-embedded stomach carcinoma using anti-Epithelial Antigen (Ep-CAM) (C-term) RabMAb (Cat. #AJ1263a).| Application Notes | WB:1:500~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
