Application
Figure 1: Western blot analysis using EPCAM mouse mAb against recombinant fragment of human EPCAM.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), breast cancer (C) and rectal cancer(D), using EPCAM mouse mAb with DAB staining.| Product Name | EPCAM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 700000000000 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GA733-2, M1S2, M4S1, MIC18, TACSTD1, TRO |
|---|---|
| Alternative Names | ESA KSA M4S1 MK-1 DIAR5 EGP-2 EGP40 KS1/4 MIC18 TROP1 TACSTD1 EPCAM |
| Molecular Weight(MW) | 34932 Da |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E |
| Tissue Specificity | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma |
| Cellular Localization | Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Note=Co-localizes with CLDN7 at the lateral cell membrane and tight junction |
| Entrez Gene | 4072 |
|---|---|
| Nucleotide Accession | NP_002345.2 |
Application
Figure 1: Western blot analysis using EPCAM mouse mAb against recombinant fragment of human EPCAM.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), breast cancer (C) and rectal cancer(D), using EPCAM mouse mAb with DAB staining.| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human EPCAM expressed in E. Coli. |
