Application
Western blot analysis of EphB4 (arrow) using rabbit polyclonal EphB4 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB4 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EphB4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HTK, MYK1, TYRO11 |
|---|---|
| Alternative Names | EPHB4 HTK MYK1 TYRO11 Ephrin type-B receptor 4 Hepatoma transmembrane kinase Tyrosine-protein kinase TYRO11 |
| Molecular Weight(MW) | 108270 Da |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis |
| Tissue Specificity | Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 2050 |
|---|
Application
Western blot analysis of EphB4 (arrow) using rabbit polyclonal EphB4 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB4 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
