Application
Figure 1: Western blot analysis using anti-EphB4 monoclonal antibody against extracellular domain of human EphB4(aa16-539).Application
Figure 2: Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using anti-EphB4 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | EPHB4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5B5 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HTK, MYK1, TYRO11 |
|---|---|
| Alternative Names | HTK MYK1 TYRO11 |
| Molecular Weight(MW) | 108270 Da |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis |
| Tissue Specificity | Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 2050 |
|---|---|
| Nucleotide Accession | NP_004435.3 |
Application
Figure 1: Western blot analysis using anti-EphB4 monoclonal antibody against extracellular domain of human EphB4(aa16-539).
Application
Figure 2: Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using anti-EphB4 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant extracellular fragment of human EphB4 fused with hIgGFc tag expressed in HEK293 cell line. |
