Application
Western blot analysis of EPHX1 (arrow) using rabbit polyclonal EPHX1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EPHX1 gene.Application
EPHX1 Antibody (N-term) western blot analysis in NCI-H292,Hela,NCI-H460 cell line lysates (35ug/lane).This demonstrates the EPHX1 antibody detected the EPHX1 protein (arrow).Application
EPHX1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EPHX1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of EPHX1 Antibody (N-term) (Cat#AP12223a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | EPHX1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EPHX, EPOX |
|---|---|
| Alternative Names | EPHX1 EPHX EPOX Epoxide hydrolase 1 Epoxide hydratase Microsomal epoxide hydrolase |
| Molecular Weight(MW) | 52949 Da |
| Function | Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water |
| Tissue Specificity | Found in liver. |
| Cellular Localization | Microsome membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein (Potential) |
| Entrez Gene | 2052 |
|---|---|
| Nucleotide Accession | NP_000111.1 |
Application
Western blot analysis of EPHX1 (arrow) using rabbit polyclonal EPHX1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EPHX1 gene.
Application
EPHX1 Antibody (N-term) western blot analysis in NCI-H292,Hela,NCI-H460 cell line lysates (35ug/lane).This demonstrates the EPHX1 antibody detected the EPHX1 protein (arrow).
Application
EPHX1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EPHX1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of EPHX1 Antibody (N-term) (Cat#AP12223a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
