Application
Western blot analysis of EPM2A (arrow) using rabbit polyclonal EPM2A Antibody (C-term) (Cat# AP1453b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPM2A gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with EPM2A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | EPM2A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EPM2A Laforin Lafora PTPase |
|---|---|
| Molecular Weight(MW) | 37158 Da |
| Function | Dual specificity protein phosphatase. May be involved in the control of glycogen metabolism, particularly in monitoring for and preventing the formation of poorly branched glycogen molecules (polyglucosans). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Forms a complex with NHLRC1/malin and HSP70 and this complex suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Isoform 2, an inactive phosphatase, could function as a dominant-negative regulator for the phosphatase activity of isoform 1 |
| Tissue Specificity | Expressed in heart, skeletal muscle, kidney, pancreas and brain. Isoform 4 is also expressed in the placenta |
| Cellular Localization | Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus Isoform 2: Endoplasmic reticulum. Cell membrane. Nucleus. Note=Also found in the nucleus Isoform 5: Cytoplasm. Nucleus. |
| Entrez Gene | 7957 |
|---|
Application
Western blot analysis of EPM2A (arrow) using rabbit polyclonal EPM2A Antibody (C-term) (Cat# AP1453b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPM2A gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with EPM2A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
