Application
A. Western blot analysis on MCF-7 cell lysates using anti-Phospho-EpoR (pY485) RabMAb (Cat. #AJ1265a), 1:50,000 dilution. Cells were either (A) untreated (B) treated with pervanadate| Product Name | EpoR Antibody Phospho (pY485) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y485 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2266 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000~50000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EPOR Erythropoietin receptor |
|---|---|
| Molecular Weight(MW) | 55065 Da |
| Function | Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3 May also activate the LYN tyrosine kinase |
| Tissue Specificity | Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early- stage erythroid progenitor cells |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 2057 |
|---|
Application
A. Western blot analysis on MCF-7 cell lysates using anti-Phospho-EpoR (pY485) RabMAb (Cat. #AJ1265a), 1:50,000 dilution. Cells were either (A) untreated (B) treated with pervanadate| Application Notes | WB:1:10000~50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
