Application
Western blot analysis of anti-EPRS Pab in Jurkat cell line lysates (35ug/lane). EPRS (arrow) was detected using the purified Pab (1:60 dilution).| Product Name | EPRS Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GLNS, PARS, QARS, QPRS |
|---|---|
| Alternative Names | EPRS GLNS PARS QARS QPRS Bifunctional glutamate/proline--tRNA ligase Bifunctional aminoacyl-tRNA synthetase Cell proliferation-inducing gene 32 protein Glutamate--tRNA ligase Glutamyl-tRNA synthetase Proline--tRNA ligase Prolyl-tRNA synthetase |
| Molecular Weight(MW) | 170591 Da |
| Function | Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop- containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation |
| Tissue Specificity | This EPRS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292~322 amino acids from the N-terminal region of human EPRS. |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 2058 |
|---|
Application
Western blot analysis of anti-EPRS Pab in Jurkat cell line lysates (35ug/lane). EPRS (arrow) was detected using the purified Pab (1:60 dilution).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
