Application
EPOR Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the EPOR antibody detected the EPOR protein (arrow).Application
EPOR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of EPOR Antibody (Center)(Cat#AP16681c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | EPOR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EPOR Erythropoietin receptor |
|---|---|
| Molecular Weight(MW) | 55065 Da |
| Function | Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3 May also activate the LYN tyrosine kinase |
| Tissue Specificity | Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early- stage erythroid progenitor cells |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 2057 |
|---|---|
| Nucleotide Accession | NP_000112.1 |
Application
EPOR Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the EPOR antibody detected the EPOR protein (arrow).
Application
EPOR Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of EPOR Antibody (Center)(Cat#AP16681c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
