Application
A. Western blot analysis on (A) SH-SY5Y and (B) HeLa cell lysates using anti-Epsin-1 RabMAb (Cat. #AJ1267a), dilution 1:2000.Application
B. Immunofluorescent staining of SH-SY5Y cells using anti-Epsin1 RabMAb(Cat. #AJ1267a).| Product Name | Epsin-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3023 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EPN1 Epsin-1 EPS-15-interacting protein 1 EH domain-binding mitotic phosphoprotein |
|---|---|
| Molecular Weight(MW) | 60293 Da |
| Function | Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Epsin-1 was used as an immunogen |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Nucleus (By similarity). Membrane, clathrin-coated pit (By similarity) Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity) |
| Entrez Gene | 29924 |
|---|
Application
A. Western blot analysis on (A) SH-SY5Y and (B) HeLa cell lysates using anti-Epsin-1 RabMAb (Cat. #AJ1267a), dilution 1:2000.
Application
B. Immunofluorescent staining of SH-SY5Y cells using anti-Epsin1 RabMAb(Cat. #AJ1267a).| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
