Application
Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1470d ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.Application
Western blot analysis of ERAS Antibody (N-term) (F66) in HL60 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (F66) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ERAS Antibody (N-term) (F66) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| IF:1:100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HRAS2, HRASP |
|---|---|
| Alternative Names | ERAS HRAS2 HRASP GTPase ERas Embryonic stem cell-expressed Ras |
| Molecular Weight(MW) | 25287 Da |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity) |
| Tissue Specificity | This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59~88 amino acids from the N-terminal region of human ERAS. |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity) |
| Entrez Gene | 3266 |
|---|
Application
Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1470d ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.
Application
Western blot analysis of ERAS Antibody (N-term) (F66) in HL60 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (F66) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | IF:1:100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
