Application
Fig 1. Western blot analysis on (A) MCF-7, (B) HeLa cell lysates using anti-ER beta RabMAb (Cat. #AJ1269a).Application
Fig 2. Immunofluorescent staining of HeLa cells using anti-ER beta RabMAb (Cat. #AJ1269a)| Product Name | ER beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3777 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50000~200000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ESTRB, NR3A2 |
|---|---|
| Molecular Weight(MW) | 59216 Da |
| Function | Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA- binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual |
| Tissue Specificity | Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain |
| Cellular Localization | Nucleus. |
| Entrez Gene | 2100 |
|---|
Application
Fig 1. Western blot analysis on (A) MCF-7, (B) HeLa cell lysates using anti-ER beta RabMAb (Cat. #AJ1269a).
Application
Fig 2. Immunofluorescent staining of HeLa cells using anti-ER beta RabMAb (Cat. #AJ1269a)| Application Notes | WB:1:50000~200000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
