Application
Figure 1: Western blot analysis using anti ERBB2 mAb against truncated ERBB2 recombinant protein.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast intraductal carcinama tissues (A) and breast infiltrating ductal carcinama tissues (B) showing membrane localization using anti-ERBB2 mAb with DAB staining.| Product Name | ERBB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6C2B12,9B9D8 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HER2, MLN19, NEU, NGL |
|---|---|
| Alternative Names | NEU HER2 TKR1 CD340 HER-2 |
| Molecular Weight(MW) | 137910 Da |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization |
| Tissue Specificity | Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Isoform 3: Cytoplasm. Nucleus. |
| Entrez Gene | 2064 |
|---|---|
| Nucleotide Accession | NP_004439.2 |
Application
Figure 1: Western blot analysis using anti ERBB2 mAb against truncated ERBB2 recombinant protein.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast intraductal carcinama tissues (A) and breast infiltrating ductal carcinama tissues (B) showing membrane localization using anti-ERBB2 mAb with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human ERBB2 (aa750-987) expressed in E. Coli. |
