Application
ERCC8 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the ERCC8 antibody detected the ERCC8 protein (arrow).Application
ERCC8 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERCC8 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ERCC8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CKN1, CSA |
|---|---|
| Alternative Names | ERCC8 CKN1 CSA DNA excision repair protein ERCC-8 Cockayne syndrome WD repeat protein CSA |
| Molecular Weight(MW) | 44055 Da |
| Function | Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription- coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes |
| Tissue Specificity | This ERCC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-238 amino acids from the Central region of human ERCC8. |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 1161 |
|---|---|
| Nucleotide Accession | NP_000073.1 |
Application
ERCC8 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the ERCC8 antibody detected the ERCC8 protein (arrow).
Application
ERCC8 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERCC8 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
